Speaker
Description
The growing threat of antibiotic resistance calls for effective alternatives. Antimicrobial peptides (AMPs) like temporin A and temporin F show promise due to their antiproliferative effects on microbial organisms and tumor cells [1,2]. As model systems for cellular membranes, lipid bilayers interact with specific AMPs, changing their mechanical characteristics and causing deterioration of membrane integrity [3]. Understanding these interactions is critical, as biological membranes are central to numerous cellular functions, including signaling and compartmentalization.In this work, we investigate the interactions between lipid membrane models and temporins A, F, and their analogs with antiproliferative properties [1,2]. To identify molecular interaction sites and assess changes in key physicochemical properties we employed Fourier-transform infrared spectroscopy and electrochemical impedance technique.. Our findings provide new insights into how temporins influence the molecular structure and dielectric behaviour of the lipid matrix. The reported alterations might facilitate peptide translocation across membranes, further supporting the therapeutic potential of the studied AMPs.
Key words: Temporins, lipid membranes, Fourier-transform infrared spectroscopy, Electrochemical impedance spectroscopy
References:
1. Dimitrova, D., Nemska, V., Foteva, T., et. al., Synthesis and Biological Studies of New Temporin A Analogs Containing Unnatural Amino Acids in Position 7, Pharmaceutics, Vol. 16, pp. 716 (2024)
2. Danalev, D., Borisova, D., Yaneva, S., et. al., Synthesis, in Vitro Biological Activity, Hydrolytic Stability and Docking of New Analogs of BIM-23052 Con-taining Halogenated Amino Acids, Amino Acids, Vol. 52, pp.1581–1592 (2020)
3. Vitkova, V., Antonova, K., Petkov, O., et. al., Interaction of KLAKLAK-NH2 and Analogs with Biomimetic Membrane Models, Pharmaceutics, Vol. 16, pp. 340 (2024)
Acknowledgements:
This work was supported by PVU – 58/12.12.2024 /BG-RRP-2.017-0041-C01/ “Development of photonic sensor effects at the interface solid surface/bionanocomposite for detection and control of biogenic impacts” (PhotonSensorEffect) in implementation of an investment under C2.I2: “Increasing the innovation capacity of the Bulgarian Academy of Sciences in the field of green and digital technologies”. This publication has been created with the financial support of the European Union – NextGenerationEU. The sole responsibility for the content of the document lies with ISSP-BAS and under no circumstances can it be assumed that this document reflects the official position of the European Union and MRS-BAS.
